PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS

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📄 Project Abstract

Leptadeniahastata (Pers.) Decne (Asclepiadaceae) is widely African plant species used in traditional medicine as an anti-venom, antihypertensive, anti-diabetic, analgesic and also used for catarrh and skin disease. The powdered leaves of L. hastatawas extracted with methanol using maceration method and the resulting crude methanol extract (ME) was suspended in water and fractionated into hexane (HF), chloroform (CF), ethylacetate (EF) and n-Butanol (BF). Preliminary phytochemical screening of ME revealed the presence of carbohydrates, alkaloids, flavonoids, anthraquinones, saponins, tannins, glycosides, steroids and triterpenes while (HF) revealed the presence of steroids and triterpenes nucleus. HF was step-wisely eluted in a silica gel packed column to afford ten fractions H1-H10 and fraction H4 was selected for further purification using Preparative Thin Layer Chromatography, with the solvent system of hexane: ethyl acetate (8.5 : 2.5). This resulted in the isolation of a whitish cr...

🔍 Key Research Areas Covered
  • ✅ Literature Review & Theoretical Framework
  • ✅ Research Methodology & Data Collection
  • ✅ Data Analysis & Statistical Methods
  • ✅ Findings & Results Discussion
  • ✅ Recommendations & Conclusions
  • ✅ References & Bibliography
📚 Complete Project Structure
Chapter 1: Introduction & Background
  • Problem Statement & Objectives
Chapter 2: Literature Review
  • Theoretical Framework & Related Studies
Chapter 3: Research Methodology
  • Data Collection & Analysis Methods
Chapter 4: Data Analysis & Results
  • Findings & Statistical Analysis
Chapter 5: Discussion & Conclusion
  • Recommendations & Future Research
Appendices: Supporting Documents
  • Questionnaires, Data, References
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"This project provided excellent guidance for my Pharmaceutical And Medicinal Chemistry Project Topics research. The methodology was clear and the data analysis helped me understand the proper approach."

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Full Citation:

Maria Manager Mailafiya. (). PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS. African and General Studies, 40, 14858.

Citation Formats:
APA
Maria Manager Mailafiya. (). PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS. African and General Studies, 40, 14858.
MLA
Maria Manager Mailafiya. "PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS." African and General Studies, vol. 40, , pp. 14858.
Chicago
Maria Manager Mailafiya. "PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS." African and General Studies 40 (): 14858.
Full Citation:

Maria Manager Mailafiya. (). PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS. African and General Studies, 40, 14858.

Citation Formats:
APA
Maria Manager Mailafiya. (). PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS. African and General Studies, 40, 14858.
MLA
Maria Manager Mailafiya. "PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS." African and General Studies, vol. 40, , pp. 14858.
Chicago
Maria Manager Mailafiya. "PHYTOCHEMICAL STUDIES AND EFFECT OF METHANOL LEAF EXTRACT OF LEPTADENIA HASTATA(PERS.) DECNE (ASCLEPIADACEAE) ON ACETIC ACID INDUCED WRITHES IN MICE AND VENOM OF ECHIS OCELLATUS." African and General Studies 40 (): 14858.
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