PHYTOCHEMICAL AND SOME PHARMACOLOGICAL STUDIES OF THE LEAF OF OCHNA RHIZOMATOSA (VAN TIEGH.) KEAY [OCHNACEAE].

📄 Project Abstract

The extract of the leaf of Ochna rhizomatosa (van Tiegh.) Keay [Ochnaceae], a plant used in the treatment of malaria in Northern Cameroun, was subjected to preliminary phytochemical screening. The result revealed the presence of carbohydrates, steroids, cardiac glycosides, terpenoids, flavonoids, tannins, fatty acids, proteins and saponins. Alkaloids were however absent. Column chromatography of the methanol fraction of water insoluble portion of the extract afforded compound MNC and BND. One of the eluents from the column with two spots was further subjected to gel filtration using sephadex LH20 which resulted in the isolation of BNF and more of compound MNC. The structure of these compounds were elucidated using chemical tests, spectroscopic techniques (NMR) and by comparison with reference data. MNC was identified to be a triflavonoid compound. BND was identified to be a Catechin, with IUPAC name 2-(3, 4-dihydroxy-phenyl)- chroman-3,5,7-triol and BNF was identified to be a biflavone...