MOLECULAR CHARACTERISATION OF VIBRIO SPECIES FROM UNILAG LAGOON AND MTU POND

📄 Project Abstract

Water being a necessity in our everyday lives and the major habitat of Vibrio species, Vibrio spp are the most common genera associated with water, human and even crustaceans often causing significant economic losses. Vibrios are ubiquitous in the aquatic environment and are commonly present in or on shellfish and other seafood or other environmentally related food or water sources. In this study two water samples were collected from two different locations (MTU pond and UNILAG lagoon) and plated on 50 different plates this was examined for the presence and absence of Vibrio species. The water samples were enriched into alkaline peptone water then inoculated into Thiosulfate citrate bile salt sucrose (TCBS) agar medium. After incubation of the 50 TCBS plates for 24hrs at 370C, the colonies were screened out for biochemical identification and confirmed using 16S rRNA PCRs. To the best of my knowledge, this is the first study done on determining the presence of potential Vibrio species i...