ISOLATION, SCREENING AND PRODUCTION OF LIPASE FROM BACILLUS SUBTILIS

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📄 Project Abstract

The production of commercial enzymes, including lipase from bacteria has always been the industrial choice due to its economic and commercial feasibility. In this study, bacterial isolates from diesel and wastewater polluted soil were screened for lipase production on solid agar. Morphological and biochemical characteristics of the best isolate were investigated. The effect of carbon sources including monosaccharide (glucose, and galactose), disaccharide (maltose, lactose, and sucrose), polysaccharide (starch), alcohol sugar (mannitol) and nitrogen sources were investigated. A total of 14 bacteria were isolated. Lipase activity ranged from 1.5f - 5.0; 2.5g - 6.0a and 3.5g - 9.5a at 24, 48 and 72 hrs respectively. Isolate PMD1 identified as Bacillus subtilis gave the best lipase activity in all the incubation periods. There was significant difference (p0.005) in the presence of different carbon and nitrogen sources employed for lipase production. Lipase production ranged from 31.09h - 8...

🔍 Key Research Areas Covered
  • ✅ Literature Review & Theoretical Framework
  • ✅ Research Methodology & Data Collection
  • ✅ Data Analysis & Statistical Methods
  • ✅ Findings & Results Discussion
  • ✅ Recommendations & Conclusions
  • ✅ References & Bibliography
📚 Complete Project Structure
Chapter 1: Introduction & Background
  • Problem Statement & Objectives
Chapter 2: Literature Review
  • Theoretical Framework & Related Studies
Chapter 3: Research Methodology
  • Data Collection & Analysis Methods
Chapter 4: Data Analysis & Results
  • Findings & Statistical Analysis
Chapter 5: Discussion & Conclusion
  • Recommendations & Future Research
Appendices: Supporting Documents
  • Questionnaires, Data, References
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📊 Complete Data

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100% original research with proper citations

📝 Properly Formatted

APA/MLA formatting with table of contents

🎓 Supervisor Approved

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Full Citation:

AYOOLA, PAMILERIN OPEYEMI. (). ISOLATION, SCREENING AND PRODUCTION OF LIPASE FROM BACILLUS SUBTILIS. African and General Studies, 40, 14858.

Citation Formats:
APA
AYOOLA, PAMILERIN OPEYEMI. (). ISOLATION, SCREENING AND PRODUCTION OF LIPASE FROM BACILLUS SUBTILIS. African and General Studies, 40, 14858.
MLA
AYOOLA, PAMILERIN OPEYEMI. "ISOLATION, SCREENING AND PRODUCTION OF LIPASE FROM BACILLUS SUBTILIS." African and General Studies, vol. 40, , pp. 14858.
Chicago
AYOOLA, PAMILERIN OPEYEMI. "ISOLATION, SCREENING AND PRODUCTION OF LIPASE FROM BACILLUS SUBTILIS." African and General Studies 40 (): 14858.
Full Citation:

AYOOLA, PAMILERIN OPEYEMI. (). ISOLATION, SCREENING AND PRODUCTION OF LIPASE FROM BACILLUS SUBTILIS. African and General Studies, 40, 14858.

Citation Formats:
APA
AYOOLA, PAMILERIN OPEYEMI. (). ISOLATION, SCREENING AND PRODUCTION OF LIPASE FROM BACILLUS SUBTILIS. African and General Studies, 40, 14858.
MLA
AYOOLA, PAMILERIN OPEYEMI. "ISOLATION, SCREENING AND PRODUCTION OF LIPASE FROM BACILLUS SUBTILIS." African and General Studies, vol. 40, , pp. 14858.
Chicago
AYOOLA, PAMILERIN OPEYEMI. "ISOLATION, SCREENING AND PRODUCTION OF LIPASE FROM BACILLUS SUBTILIS." African and General Studies 40 (): 14858.
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