In Vivo And In Vitro Studies Of Ocimum Basilicum [Labiata] Leaf Extracts On Intestinal Motility

📄 Project Abstract

Ocimum basilicum is a dicotyledonous shrub grown in most parts of Nigeria. Its medicinal properties had gained popularity in traditional medicine. In addition the leaves are eaten in soups in many homes. It consists of a well defined mixture of flavonoids, vitamins, amino acids, sugars, anions, linalool, tannins, rosmarinic acid, salicylic acid, essential oils, fats and terpenes. The acute oral toxicity [determination of median lethal dose (LD50)], in vitro and in vivo experiments of aqueous and methanolic extracts were done. Acute toxicity test of aqueous and methanolic extracts of Ocimum basilicum was carried out on mice. In this study, fasted mice were given 10, 100, 1000, 1600, 2900 and 5000 mg/Kg of both extracts. Median lethal dose (LD50) was 3807.89 and 2154.07 mg/Kg for aqueous and methanolic extracts respectively. The study to determine in vivo effect of Ocimum basilicum leaf extracts of the shrub was on the gastrointestinal transit time in conscious rats. In the in vivo exper...