Genetics Of Resistance To Aspergillus Flavus Link Ex Fries In Groundnut (Arachishypogaea L

📄 Project Abstract

The mode of inheritance to surface seed colonization by Aspergillusflavus Link ex Fries and aflatoxin contamination in groundnut (Arachishypogaea L.) was studied from four groundnut varieties. Two varieties were reported to be resistant (J11 and 55-437) and the remaining two susceptible (Samnut23 and Samnut 22). Each resistant variety was crossed to the two susceptible varieties in a bi-parental mating design in the screen house of the Institute for Agricultural Research, Ahmadu Bello University, Samaru Zaria. Six genetic populations (P1, P2, F1, F2, BCP1 and BCP2) were developed which were evaluated for surface seeds colonization by inoculation with conidia of Aspergillusflavus in the laboratory. The inoculated seeds were incubated at 28oCfor 8 days, at the end of which the seeds were rated for surface seeds colonization. Aflatoxins were extracted from the inoculated seeds by column chromatography and analysed for aflatoxin content by thin layer chromatography (TLC). The data collecte...