EVALUATION OF CRUDE OIL CONTAMINATED SOIL AFTER BIOREMEDIATION AND BIOCEMENTATION USING BACILLUS LICHENIFORMIS FOR USE IN WASTE CONTAINMENT APPLICATIONS

📄 Project Abstract

A study was conducted in the laboratory on a crude oil contaminated soil treated with Bacillus licheniformis to investigate the suitability of the material for use as a liner material in waste containment applications. An indigenous hydrocarbon utilizing bacteria (HUB) was identified as Bacillus licheniformis and isolated from crude oil contaminated soil (COCS) collected from the Nigerian National Petroleum Corporation (NNPC) Kaduna, Nigeria, at a depth of 0.5m from the surface. A bio-stimulation strategy of bio-remediation was adopted using chicken droppings (CD) in stepped concentrations of 0, 10, 20, 30 and 40% CD content as a source of nutrients only, to activate the indigenous bacteria for a 12 weeks period of remediation. At the end of the remediation, it was observed that Bacillus licheniformis removed 89.8% of crude oil content out of the initial 98g/kg of total petroleum hydrocarbon concentration at 30% CD dosage. The bio-remediated soil was further subjected to microbial acti...