DEVELOPMENT AND VALIDATION OF THREE NEW SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF LAMIVUDINE IN PURE AND PHARMACEUTICAL DOSAGE FORMS

📄 Project Abstract

In this study three rapid, simple, accurate, economical and reproducible spectrophotometric methods for the quantitative determination of lamivudine in pure form and tablet formulations were developed and validated. The first method is based on dissolution of standard lamivudine powder or extraction of the drug from tablet formulation using methanol as solvent. The resulting extract was filtered and scanned using Helios Zeta Model 164617 UV/Vis spectrophotometer; having a max of 273 nm. The second method is based on the formation of a coloured hydrazone by reacting the hydrazine group in 2,4-dinitrophenyl hydrazine with the carbonyl carbon in lamivudine under acidic condition (85 % H2SO4) for 10 minutes. The orange-red coloured hydrazone formed was allowed to stand for 2 hours for complete colour development, then scanned using spectrophotometer and was observed to have a max of 438 nm. The third method is based on the diazotization reaction of lamivudine using sodium nitrite and sulfa...