DEVELOPMENT AND VALIDATION OF FOUR NEW UVSPECTROMETRIC METHODS FOR THE DETERMINATION OF ISONIAZID (INH) IN PURE AND TABLET DOSAGE FORMS

📄 Project Abstract

Tuberculosis (TB) is a serious infectious disease which is on increase since emergence of human immunodeficiency virus (HIV) leading to TB/HIV co infection, causing complications and treatment failure. Isoniazid is a first line drug for the treatment of TB therefore it is important to develop methods that will monitor its quality. In this study, four rapid, simple, relatively accurate, and precise spectrophotometric methods for the quantitative determination of isoniazid in pure and tablet dosage form were developed and validated. Methods 1 and 2 were based on dissolving standard isoniazid powder in distilled water and methanol respectively and scanning using UV-Vis spectrophotometer at a wavelength range of 200 - 400 nm to determine the wavelength of maximum absorption (max). Method 3 was based on condensation reaction between isoniazid and 2,4-di-nitrophenyl hydrazine (2,4 DNPH) and heating under acidic condition using 2 mL of 85 % hydrochloric acid (HCl) to form an orange-red hydraz...