Comparative Study Of The Effect Of Table Salt In 10% Formal Saline And Saturated Sodium Chloride Solution As Tissue Fixatives

📄 Project Abstract

Formal saline and saturated sodium chloride solutions were evaluated and compared as tissue fixatives in a quest of finding a safer and cheaper alternative to formaldehyde. Livers and testes dissected from 20 male rats allotted to 4 groups of 5 animals each were fixed for 12hrs, 24hrs, 48hrs and 7days in either standard formal saline (control) or formal saline prepared with table salt or saturated sodium chloride solution prepared with laboratory salt or saturated sodium chloride solution prepared with table salt and labeled as groups A, B, C and D respectively. The livers and testes were processed routinely and stained with H&E. The histological features (such as good staining, morphological appearance of the nucleus and cytoplasm etc) observed in the liver sections were not significantly different across the fixative groups at all fixative periods while those observed in the testes sections fixed in formal saline (both the standard and the one prepared with table salt) were significa...