Assessment Of Cytologic Diagnosis Of Childhood Non Hodgkin Lymphoma Using Immunocytochemistry In Oauthc, Ile-Ife.

📄 Project Abstract

Burkitt lymphoma (BL) is the commonest childhood cancer in Nigeria, and tissue morphology has remained the standard diagnostic method. However, most clinicians in sub-Saharan Africa still rely solely on cytology for the diagnosis of BL because it is quick and inexpensive. Distinguishing BL from other confounding childhood tumours, using cytology remains a challenge. This study was designed to assess the validity of cytology in the diagnosis of BL, using immunocytochemistry as the gold standard. This study was both prospective and retrospective. Eighty-four subjects were recruited using a convenient sampling technique but only 50 cases had complete data; 41 prospective and nine retrospective cases. Immunocytochemistry using BCL-2 and Ki-67 was done. Burkitt lymphoma was diagnosed when BCL-2 was negative with Ki-67 grade of at least 90%. Using cytology with Leishman staining, 33 (66%) were diagnosed with BL, 17 (34%) nonBL (16 lymphoblastic lymphoma/leukaemia and 1 unclassifiable). Of th...